Expression and biochemical characterization of human immunodeficiency virus type 1 nef gene product
نویسندگان
چکیده
منابع مشابه
Inhibition of protein synthesis by the human immunodeficiency virus type 1 nef gene product.
During productive infection of human T lymphocytes in cell culture, the expression of human immunodeficiency virus type 1 is temporally regulated by virus-encoded regulatory proteins. Among these Nef, whose function has not been clearly elucidated, is thought to alter CD4+ T cells. We examined the possibility that the nef gene interferes with the translation process in a cell-free system. The r...
متن کاملQuantification Analysis of Dot Blot Assays for Human Immunodeficiency Virus Type 1 and 2 Antibodies
Objective Dot Blot (DB) assay provides highly specific results, but usually not reliable for quantification of antibody production. The need for a more objective DB assay to provide a better definition of the immune status, against HIV antigens, promoted this study to be done to develop a quantitative DB assay. Material and Methods Dot blot (DB) strips for antibodies directed to human immuno...
متن کاملIntra-host competition between nef-defective escape mutants and wild-type human immunodeficiency virus type 1.
Various forms of nef genes with deletions at conserved positions along the sequence have been reported to persist in human immunodeficiency virus type 1 infected patients. We investigate the forces maintaining such variants in the proviral population. The main selection pressures are preservation of function and host immune response. The crippled Nef protein might have fewer epitopes, and as su...
متن کاملResistance mechanism of human immunodeficiency virus type-1 protease to inhibitors: A molecular dynamic approach
Human immunodeficiency virus type 1 (HIV-1) protease inhibitors comprise an important class of drugs used in HIV treatments. However, mutations of protease genes accelerated by low fidelity of reverse transcriptase yield drug resistant mutants of reduced affinities for the inhibitors. This problem is considered to be a serious barrier against HIV treatment for the foreseeable future. In this st...
متن کاملBiochemical Characterization of a Recombinant TRIM5" Protein That Restricts Human Immunodeficiency Virus Type 1 Replication†
Biochemical Characterization of a Recombinant TRIM5" Protein That Restricts Human Immunodeficiency Virus Type 1 Replication† Charles R. Langelier, Virginie Sandrin, Debra M. Eckert, Devin E. Christensen, Viswanathan Chandrasekaran, Steven L. Alam, Christopher Aiken, John C. Olsen, Alak Kanti Kar, Joseph G. Sodroski, and Wesley I. Sundquist* Department of Biochemistry, University of Utah, Salt L...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1990
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.64.7.3447-3454.1990